Inhibition of Choriocapillaris Regeneration Genistein

METHODS. Intravitreal injections of either 0.1 ml of a 90-JLLM concentration of genistein, dimethyl sulfoxide (DMSO; negative control), or 2 fxg cycloheximide (positive control) were given 24 hours before surgical debridement of RPE in rabbits. Scanning electron microscopy (EM) of choroidal vascular casts and the RPE wounds and histologic evaluation by light microscopy and EM of the disturbed areas were performed at days 1, 7, and 30 after surgery. Quantitative analysis of the area of the choriocapillaris bed and RPE was performed by automated image analysis, and the results were analyzed by paired Student's Mest.